Common and phylogenetically widespread coding for peptides by bacterial small RNAs.

ABSTRACT:

Background

While eukaryotic noncoding RNAs have recently received intense scrutiny, it is becoming clear that bacterial transcription is at least as pervasive. Bacterial small RNAs and antisense RNAs (sRNAs) are often assumed to be noncoding, due to their lack of long open reading frames (ORFs). However, there are numerous examples of sRNAs encoding for small proteins, whether or not they also have a regulatory role at the RNA level.

Methods

Here, we apply flexible machine learning techniques based on sequence features and comparative genomics to quantify the prevalence of sRNA ORFs under natural selection to maintain protein-coding function in 14 phylogenetically diverse bacteria. Importantly, we quantify uncertainty in our predictions, and follow up on them using mass spec

SUBMITTER: Friedman RC 

PROVIDER: S-EPMC5521070 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature