GlycoPep MassList: software to generate massive inclusion lists for glycopeptide analyses.

ABSTRACT: Protein glycosylation drives many biological processes and serves as markers for disease; therefore, the development of tools to study glycosylation is an essential and growing area of research. Mass spectrometry can be used to identify both the glycans of interest and the glycosylation sites to which those glycans are attached, when proteins are proteolytically digested and their glycopeptides are analyzed by a combination of high-resolution mass spectrometry (MS) and tandem mass spectrometry (MS/MS) methods. One major challenge in these experiments is collecting the requisite MS/MS data. The digested glycopeptides are often present in complex mixtures and in low abundance, and the most commonly used approach to collect MS/MS data on these species is data-dependent acquisition (DDA), wher

SUBMITTER: Hu W 

PROVIDER: S-EPMC5521198 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature