Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase.

ABSTRACT: Xylanases are important polysaccharide-cleaving catalysts for the pulp and paper, animal feeds and biofuels industries. They have also proved to be valuable model systems for understanding enzymatic catalysis, with one of the best studied being the GH11 xylanase from Bacillus circulans (Bcx). However, proteins from this class are very recalcitrant to refolding in vitro. This both limits their high level expression in heterologous hosts, and prevents experimental approaches, such as peptide ligation or chemical modifications, to probe and engineer their stability and function. To solve this problem, a systematic screening approach was employed to identify suitable buffer conditions for renaturing Bcx in vitro. The fractional factorial screen employed identified starting conditions for refol

SUBMITTER: Kotzler MP 

PROVIDER: S-EPMC5521550 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature