A rapid expression and purification condition screening protocol for membrane protein structural biology.

ABSTRACT: Membrane proteins control a large number of vital biological processes and are often medically important-not least as drug targets. However, membrane proteins are generally more difficult to work with than their globular counterparts, and as a consequence comparatively few high-resolution structures are available. In any membrane protein structure project, a lot of effort is usually spent on obtaining a pure and stable protein preparation. The process commonly involves the expression of several constructs and homologs, followed by extraction in various detergents. This is normally a time-consuming and highly iterative process since only one or a few conditions can be tested at a time. In this article, we describe a rapid screening protocol in a 96-well format that largely mimics standard m

SUBMITTER: Sjostrand D 

PROVIDER: S-EPMC5521553 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature