Radiation improves antitumor effect of immune checkpoint inhibitor in murine hepatocellular carcinoma model.

ABSTRACT:

Background & aims

Although immunotherapy has emerged as an attractive therapy for refractory cancers, its limited efficacy in hepatocellular carcinoma (HCC) suggests the need for a combination strategy that can either enhance or complement therapeutic effect. We investigated whether combination of immune checkpoint blockade (ICB) and radiation could enhance antitumor effect in a murine HCC model.

Methods

Using murine HCC, HCa-1, the effect of radiation on programmed death-ligand1 (PD-L1) expression was determined by real-time PCR, flow cytometry, and western blotting. Signaling pathways involved in altered PD-L1 expression were examined. Tumor growth and survival rate were evaluated for a combination of anti-PD-L1 and radiation. Immunological parameters in the tumor were ass

SUBMITTER: Kim KJ 

PROVIDER: S-EPMC5522235 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature