Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate.

ABSTRACT: Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H₂. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H₂ production and formate consumption increased remarkably. The 156 times transferred-strain, WTF-156T, was demonstrated to enhance H₂ production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was foun

SUBMITTER: Jung HC 

PROVIDER: S-EPMC5522443 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature