Epigenetic memory of oxidative stress: does nephrilin exert its protective effects via Rac1?

ABSTRACT:

Aim

Nephrilin peptide, a designed inhibitor of Rictor complex (mTORC2), exerts pleiotropic protective effects in metabolic, xenobiotic and traumatic stress models. Stress can generate enduring epigenetic changes in gene function. In this work we examine the possibility that nephrilin treatment protects against acute and enduring global changes in oxidative metabolism, with a focus on the Rictor-complex-mediated activation of Rac1, a subunit of NADPH oxidase (Nox) via PKCs, Prex1 and p66shc.

Methods

Given the wide range of animal models in which nephrilin peptide has previously demonstrated effectiveness in vivo, we chose three different experimental systems for this investigation: dermal fibroblasts, renal proximal tubule epithelial cells (PTECs), and kidney tissue and urine

SUBMITTER: Mascarenhas DD 

PROVIDER: S-EPMC5522666 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature