Quantifying transcription factor binding dynamics at the single-molecule level in live cells.

ABSTRACT: Progressive, technological achievements in the quantitative fluorescence microscopy field are allowing researches from many different areas to start unraveling the dynamic intricacies of biological processes inside living cells. From super-resolution microscopy techniques to tracking of individual proteins, fluorescence microscopy is changing our perspective on how the cell works. Fortunately, a growing number of research groups are exploring single-molecule studies in living cells. However, no clear consensus exists on several key aspects of the technique such as image acquisition conditions, or analysis of the obtained data. Here, we describe a detailed approach to perform single-molecule tracking (SMT) of transcription factors in living cells to obtain key binding characteristics, namel

SUBMITTER: Presman DM 

PROVIDER: S-EPMC5522764 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature