Comparative analysis of 2D and 3D distance measurements to study spatial genome organization.

ABSTRACT: The spatial organization of genomes is non-random, cell-type specific, and has been linked to cellular function. The investigation of spatial organization has traditionally relied extensively on fluorescence microscopy. The validity of the imaging methods used to probe spatial genome organization often depends on the accuracy and precision of distance measurements. Imaging-based measurements may either use 2 dimensional datasets or 3D datasets which include the z-axis information in image stacks. Here we compare the suitability of 2D vs 3D distance measurements in the analysis of various features of spatial genome organization. We find in general good agreement between 2D and 3D analysis with higher convergence of measurements as the interrogated distance increases, especially in flat cell

SUBMITTER: Finn EH 

PROVIDER: S-EPMC5522766 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature