In vitro-in silico-based analysis of the dose-dependent in vivo oestrogenicity of the soy phytoestrogen genistein in humans.

ABSTRACT:

Background and purpose

The in vivo oestrogenicity of genistein and its glycoside genistin is still under debate. The present study aimed to develop a physiologically based kinetic (PBK) model that provides insight in dose-dependent plasma concentrations of genistein aglycone and its metabolites and enables prediction of in vivo oestrogenic effective dose levels of genistein and genistin in humans.

Experimental approach

A PBK model for genistein and genistin in humans was developed based on in vitro metabolic parameters. The model obtained was used to translate in vitro oestrogenic concentration-response curves of genistein to in vivo oestrogenic dose-response curves for intake of genistein and genistin.

Key results

The model predicted that genistein-7-O-glucuronide wa

SUBMITTER: Boonpawa R 

PROVIDER: S-EPMC5523000 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature