Cytoplasmic Dynein Transports Axonal Microtubules in a Polarity-Sorting Manner.

ABSTRACT: Axonal microtubules are predominantly organized into a plus-end-out pattern. Here, we tested both experimentally and with computational modeling whether a motor-based polarity-sorting mechanism can explain this microtubule pattern. The posited mechanism centers on cytoplasmic dynein transporting plus-end-out and minus-end-out microtubules into and out of the axon, respectively. When cytoplasmic dynein was acutely inhibited, the bi-directional transport of microtubules in the axon was disrupted in both directions, after which minus-end-out microtubules accumulated in the axon over time. Computational modeling revealed that dynein-mediated transport of microtubules can establish and preserve a predominantly plus-end-out microtubule pattern as per the details of the experimental findings, but

SUBMITTER: Rao AN 

PROVIDER: S-EPMC5523108 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature