In vivo assessment of intracellular redox state in rat liver using hyperpolarized [1-¹³ C]Alanine.

ABSTRACT:

Purpose

The intracellular lactate to pyruvate concentration ratio is a commonly used tissue assay biomarker of redox, being proportional to free cytosolic [NADH]/[NAD⁺ ]. In this study, we assessed the use of hyperpolarized [1-¹³ C]alanine and the subsequent detection of the intracellular products of [1-¹³ C]pyruvate and [1-¹³ C]lactate as a useful substrate for assessing redox levels in the liver in vivo.

Methods

Animal experiments were conducted to measure in vivo metabolism at baseline and after ethanol infusion. A solution of 80-mM hyperpolarized [1-¹³ C]alanine was injected intravenously at baseline (n = 8) and 45 min after ethanol infusion (n = 4), immediately followed by the dynamic acquisition of ¹³ C

SUBMITTER: Park JM 

PROVIDER: S-EPMC5523144 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature