Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein.

ABSTRACT: Babesiosis caused by Babesia orientalis is one of the most prevalent infections of water buffalo transmitted by Rhipicephalus haemaphysaloides causing a parasitic and hemolytic disease. The organelles proteins localized in apical membrane especially rhoptries neck and microneme protein form a complex called moving junction important during invasion process of parasites belonging to apicomplexan group, including Babesia species. A truncated fragment coding a 936 bps fragment was cloned in pMD-19T and subcloned into pET32 (a)⁺ expression vector, expressed in E. coli BL21. Purified recombinant BoRON2 was used to produce polyclonal antibody against BoRON2. Here, we identified the full sequence of gene encoding the rhoptry neck 2 protein that

SUBMITTER: Malobi N 

PROVIDER: S-EPMC5523350 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature