Structural and Biophysical Characterization of Cajanus cajan Protease Inhibitor.

ABSTRACT:

Context

A large number of studies have proven that Protease inhibitors (PIs), specifically serine protease inhibitors, show immense divergence in regulation of proteolysis by targeting their specific proteases and hence, they play a key role in healthcare.

Objective

We aimed to access in-vitro anticancer potential of PI from Cajanus cajan (CCPI). Also, crystallization of CCPI was targetted alongwith structure determination and its structure-function relationship.

Materials and methods

CCPI was purified from Cajanus cajan seeds by chromatographic techniques. The purity and molecular mass was determined by SDS-PAGE. Anticancer potential of CCPI was determined by MTT assay in normal HEK and cancerous A549 cells. The crystallization screening of CCPI

SUBMITTER: Shamsi TN 

PROVIDER: S-EPMC5523526 | biostudies-literature | 2017 Jul-Dec

REPOSITORIES: biostudies-literature