Cigarette smoke impairs cytokine responses and BCG containment in alveolar macrophages.

ABSTRACT:

Background

There is a strong epidemiological link between smoking and tuberculosis (TB), but the association is confounded by socioeconomic and other factors. A direct relationship between cigarette smoke and poor treatment-related outcomes in patients with TB is therefore questionable. We investigated whether constituents of tobacco smoke impair mycobacterial host immune responses in vitro.

Methodology

Preparation of a cigarette smoke extract (CSE) from Marlboro Red cigarettes was standardised and reproducibility verified by mass spectroscopy. Macrophages were derived from peripheral blood monocytes (MDM) and alveolar macrophages from bronchoalveolar lavage fluid from healthy non-smoking volunteers. Mycobacterial uptake (flow cytometric detection of fluorescence using green

SUBMITTER: van Zyl-Smit RN 

PROVIDER: S-EPMC5523928 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature