Affinity of human IgG subclasses to mouse Fc gamma receptors.

ABSTRACT: Human IgG is the main antibody class used in antibody therapies because of its efficacy and longer half-life, which are completely or partly due to Fc?R-mediated functions of the molecules. Preclinical testing in mouse models are frequently performed using human IgG, but no detailed information on binding of human IgG to mouse Fc?Rs is available. The orthologous mouse and human Fc?Rs share roughly 60-70% identity, suggesting some incompatibility. Here, we report binding affinities of all mouse and human IgG subclasses to mouse Fc?R. Human IgGs bound to mouse Fc?R with remarkably similar binding strengths as we know from binding to human ortholog receptors, with relative affinities IgG3>IgG1>IgG4>IgG2 and Fc?RI>>Fc?RIV>Fc?RIII>Fc?RIIb. This suggests human IgG subclasses to have similar relative Fc?R-mediated biological activities in mice.

SUBMITTER: Dekkers G 

PROVIDER: S-EPMC5524164 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature