Boc₃Arg-Linked Ligands Induce Degradation by Localizing Target Proteins to the 20S Proteasome.

ABSTRACT: Targeted protein degradation is a promising strategy for drug design and functional assessment. Several small molecule approaches have been developed that localize target proteins to ubiquitin ligases, inducing ubiquitination and subsequent degradation by the 26S proteasome. We discovered that the degradation of a target protein can also be induced by a recognition ligand linked to tert-butyl carbamate (Boc₃)-protected arginine (B₃A). Here, we show that this process requires the proteasome but does not involve ubiquitination of the target protein. B₃A does not perturb the structure of the target protein; instead, a B₃A-ligand stabilizes its target protein. B₃A ligands stimulate activity of purified 20S proteasome, demonstrating that th

SUBMITTER: Shi Y 

PROVIDER: S-EPMC5524191 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature