Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation.

ABSTRACT: Tamm-Horsfall protein (THP) is an abundant urinary protein of renal origin. We hypothesize that THP can act as an inhibitor of complement since THP binds complement 1q (C1q) of the classical complement pathway, inhibits activation of this pathway, and is important in decreasing renal ischemia-reperfusion injury (a complement-mediated condition). In this study, we began to investigate whether THP interacted with the alternate complement pathway via complement factor H (CFH). THP was shown to bind CFH using ligand blots and in an ELISA (KD of 1 × 10-6 M). Next, the ability of THP to alter CFH's normal action as it functioned as a cofactor in complement factor I (CFI)-mediated complement 3b (C3b) degradation was investigated. Unexpectedly, control experiments in these in vitro assays suggeste

SUBMITTER: Rhodes DCJ 

PROVIDER: S-EPMC5524369 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature