Differential protein expression and basal lamina remodeling in human heart failure.

ABSTRACT:

Purpose

A goal of this study was to identify and investigate previously unrecognized components of the remodeling process in the progression to heart failure by comparing protein expression in ischemic failing (F) and nonfailing (NF) human hearts.

Experimental design

Protein expression differences were investigated using multidimensional protein identification and validated by Western analysis. This approach detected basal lamina (BL) remodeling, and further studies analyzed samples for evidence of structural BL remodeling. A rat model of pressure overload (PO) was studied to determine whether nonischemic stressors also produce BL remodeling and impact cellular adhesion.

Results

Differential protein expression of collagen IV, laminin α2, and nidogen-1 indicated BL rem

SUBMITTER: Kim EH 

PROVIDER: S-EPMC5524561 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature