PLATE-Seq for genome-wide regulatory network analysis of high-throughput screens.

ABSTRACT: Pharmacological and functional genomic screens play an essential role in the discovery and characterization of therapeutic targets and associated pharmacological inhibitors. Although these screens affect thousands of gene products, the typical readout is based on low complexity rather than genome-wide assays. To address this limitation, we introduce pooled library amplification for transcriptome expression (PLATE-Seq), a low-cost, genome-wide mRNA profiling methodology specifically designed to complement high-throughput screening assays. Introduction of sample-specific barcodes during reverse transcription supports pooled library construction and low-depth sequencing that is 10- to 20-fold less expensive than conventional RNA-Seq. The use of network-based algorithms to infer protein activi

SUBMITTER: Bush EC 

PROVIDER: S-EPMC5524642 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature