AGC family kinase 1 participates in trogocytosis but not in phagocytosis in Entamoeba histolytica.

ABSTRACT: The protozoan parasite Entamoeba histolytica is the aetiologic agent of amoebiasis, an endemic infection in developing countries with considerable morbidity and mortality. Recently, trogocytosis has been recognized as the key step in amoebic cytolysis and invasion, a paradigm shift in understanding pathogenicity of this organism. Here we report that AGC family kinase 1 is specifically involved in trogocytosis of live human cells and does not participate in phagocytosis of dead cells. Live imaging reveals localization of this kinase in the long and thin tunnels formed during trogocytosis but not in the trogosomes (endosomes formed after trogocytosis). Silencing of the specific gene leads to a defect in CHO cell destruction and trogocytosis while other endocytic processes remain unaffected.

SUBMITTER: Somlata 

PROVIDER: S-EPMC5524646 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature