In vivo Host-Pathogen Interaction as Revealed by Global Proteomic Profiling of Zebrafish Larvae.

ABSTRACT: The outcome of a host-pathogen interaction is determined by the conditions of the host, the pathogen, and the environment. Although numerous proteomic studies of in vitro-grown microbial pathogens have been performed, in vivo proteomic approaches are still rare. In addition, increasing evidence supports that in vitro studies inadequately reflect in vivo conditions. Choosing the proper host is essential to detect the expression of proteins from the pathogen in vivo. Numerous studies have demonstrated the suitability of zebrafish (Danio rerio) embryos as a model to in vivo studies of Pseudomonas aeruginosa infection. In most zebrafish-pathogen studies, infection is achieved by microinjection of bacteria into the larvae. However, few rep

SUBMITTER: Diaz-Pascual F 

PROVIDER: S-EPMC5524664 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature