Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography.

ABSTRACT: High-throughput three-dimensional cryogenic imaging of thick biological specimens is valuable for identifying biologically- or pathologically-relevant features of interest, especially for subsequent correlative studies. Unfortunately, high-resolution imaging techniques at cryogenic conditions often require sample reduction through sequential physical milling or sectioning for sufficient penetration to generate each image of the 3-D stack. This study represents the first demonstration of using ptychographic hard X-ray tomography at cryogenic temperatures for imaging thick biological tissue in a chemically-fixed, frozen-hydrated state without heavy metal staining and organic solvents. Applied to mammalian brain, this label-free cryogenic imaging method allows visualization of myelinated axon

SUBMITTER: Shahmoradian SH 

PROVIDER: S-EPMC5524705 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature