Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently.

ABSTRACT: We conducted quantitative cellular respiration analysis on samples taken from human breast cancer (HBC) and human colorectal cancer (HCC) patients. Respiratory capacity is not lost as a result of tumor formation and even though, functionally, complex I in HCC was found to be suppressed, it was not evident on the protein level. Additionally, metabolic control analysis was used to quantify the role of components of mitochondrial interactosome. The main rate-controlling steps in HBC are complex IV and adenine nucleotide transporter, but in HCC, complexes I and III. Our kinetic measurements confirmed previous studies that respiratory chain complexes I and III in HBC and HCC can be assembled into supercomplexes with a possible partial addition from the complex IV pool. Therefore, the kinetic me

SUBMITTER: Koit A 

PROVIDER: S-EPMC5525093 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature