A Phytochrome-Derived Photoswitch for Intracellular Transport.

ABSTRACT: Cells depend on the proper positioning of their organelles, suggesting that active manipulation of organelle positions can be used to explore spatial cell biology and to restore cellular defects caused by organelle misplacement. Recently, blue-light dependent recruitment of specific motors to selected organelles has been shown to alter organelle motility and positioning, but these approaches lack rapid and active reversibility. The light-dependent interaction of phytochrome B with its interacting factors has been shown to function as a photoswitch, dimerizing under red light and dissociating under far-red light. Here we engineer phytochrome domains into photoswitches for intracellular transport that enable the reversible interaction between organelles and motor proteins. Using patterned il

SUBMITTER: Adrian M 

PROVIDER: S-EPMC5525101 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature