Improved detection of genus-specific Alphavirus using a generic TaqMan® assay.

ABSTRACT:

Background

Alphaviruses are arthropod borne RNA viruses of medical importance. Geographical expansion of mosquitoes of the Aedes genus in the past decades has been associated with major Alphavirus-associated outbreaks. Climate changes and intensification of air travels have favored vector expansion and virus dissemination in new territories leading to virus emergence not only in tropical areas but also in temperate regions. The detection of emergence is based upon surveillance networks with epidemiological and laboratory investigation.

Method

A specific, sensitive and rapid screening test for genus-specific Alphavirus is critically required. To address this issue, we developed a new molecular assay targeting nsP4 gene and using a TaqMan® real time RT-PCR method for the speci

SUBMITTER: Giry C 

PROVIDER: S-EPMC5525299 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature