Differential effects of FTY720 on the B cell compartment in a mouse model of multiple sclerosis.

ABSTRACT:

Background

MP4-induced experimental autoimmune encephalomyelitis (EAE) is a mouse model of multiple sclerosis (MS), which enables targeted research on B cells, currently much discussed protagonists in MS pathogenesis. Here, we used this model to study the impact of the S1P₁ receptor modulator FTY720 (fingolimod) on the autoreactive B cell and antibody response both in the periphery and the central nervous system (CNS).

Methods

MP4-immunized mice were treated orally with FTY720 for 30 days at the peak of disease or 50 days after EAE onset. The subsequent disease course was monitored and the MP4-specific B cell/antibody response was measured by ELISPOT and ELISA. RNA sequencing was performed to determine any effects on B cell-relevant gene expression. S1P₁

SUBMITTER: Bail K 

PROVIDER: S-EPMC5525315 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature