Genome-wide DNA methylation profile analysis identifies differentially methylated loci associated with ankylosis spondylitis.

ABSTRACT:

Background

Ankylosing spondylitis (AS) is a chronic rheumatic and autoimmune disease. Little is known about the potential role of DNA methylation in the pathogenesis of AS. This study was undertaken to explore the potential role of DNA methylation in the genetic mechanism of AS.

Methods

In this study, we compared the genome-wide DNA methylation profiles of peripheral blood mononuclear cells (PBMCs) between five AS patients and five healthy subjects, using the Illumina Infinium HumanMethylation450 BeadChip. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed to validate the relevance of the identified differentially methylated genes for AS, using another independent sample of five AS patients and five healthy subjects.

Results

SUBMITTER: Hao J 

PROVIDER: S-EPMC5526246 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature