Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis).

ABSTRACT: Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis of Rhus chinensis. S. chinensis has a complex life cycle, with a switch of hosts between R. chinensis and certain mosses, and a switch of sexual and asexual reproduction (cyclical parthenogenesis). We have cloned a full-length cDNA of the β-tubulin gene from S. chinensis, using qPCR and RACE. This cDNA has 1606 base pairs with a 251 bp 5'-untranslated region (5'-UTR) and a 15 bp 3'-untranslated region (3'-UTR). The gene encodes a protein with 376 amino acids residues. The expression levels of the β-tubuli

SUBMITTER: Liu P 

PROVIDER: S-EPMC5526861 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature