Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray.

ABSTRACT: There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platform. Avian egg white (EW) provides nutrition for the embryo and protects against infection, with glycosylation responsible for binding certain pathogens. In this study, a microarray platform of 78 species of avian EWs was developed and profiled for glycosylation using a lectin panel with a wide range of carbohydrate specificities. The dominating linkages of sialic acid in EWs were determined for the first time using the lectins MAA and SNA-I. EW glycosylation similarity among the different orders of bird

SUBMITTER: Utratna M 

PROVIDER: S-EPMC5526940 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature