Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids.

ABSTRACT: Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories. Here, we present a novel and complementary approach that utilizes essential microbial glycolipids as chemical fingerprints for identification of individual bacterial species. Gram-positive and negative bacterial glycolipids were extracted using a

SUBMITTER: Leung LM 

PROVIDER: S-EPMC5526941 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature