Parasitological, Serological and Molecular Study of Dirofilaria immitis in Domestic Dogs, Southeastern Iran.

ABSTRACT:

Background

Dirofilariasis is a serious and potentially deadly condition in dogs and one of the zoonotic filarial infections, which inadvertently affects the humans. The objectives of this study were to determine the seroprevalence and the molecular identity of dirofilariasis in Kerman Province, southeastern Iran between Jul and Aug 2013.

Methods

A hundred and forty-nine domestic dogs were randomly selected and five ml blood samples were taken from each dog. One ml of anticoagulant (EDTA) was used for each test in the parasitological study (modified Knott's test) and sera samples were examined, using ELISA kit to detect Dirofilaria immitis antigen. Extracted DNA of all positive blood samples was used for molecular characterization and sequencing.

Results

Four (2

SUBMITTER: Bamorovat M 

PROVIDER: S-EPMC5527037 | biostudies-literature | 2017 Apr-Jun

REPOSITORIES: biostudies-literature