Inactivation of DNase1L2 and DNase2 in keratinocytes suppresses DNA degradation during epidermal cornification and results in constitutive parakeratosis.

ABSTRACT: The stratum corneum of the epidermis constitutes the mammalian skin barrier to the environment. It is formed by cornification of keratinocytes, a process which involves the removal of nuclear DNA. Here, we investigated the mechanism of cornification-associated DNA degradation by generating mouse models deficient of candidate DNA-degrading enzymes and characterizing their epidermal phenotypes. In contrast to Dnase1l2 ^-/- mice and keratinocyte-specific DNase2 knockout mice (Dnase2 ^Δep ), Dnase1l2 ^-/- Dnase2 ^Δep mice aberrantly retained nuclear DNA in the stratum corneum, a phenomenon commonly referred to as parakeratosis. The DNA within DNase1L2/DNase2-deficient corneocytes was partially degraded in a DNase1-independent manner. Isolation of corneoc

SUBMITTER: Fischer H 

PROVIDER: S-EPMC5527052 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature