Urinary sampling for 5HIAA and metanephrines determination: revisiting the recommendations.

ABSTRACT:

Context

Biogenic amines such as 5-hydroxy-indole acetic acid (5HIAA) the main metabolite of serotonin or metanephrines (catecholamines metabolites) are used as biomarkers of neuroendocrine tumours.

Objective

To re-evaluate the recommendations for urinary sampling (preservatives, diet, drugs, etc.) as many of the reported analytical interferences supporting these recommendations are related to obsolete assays.

Methods

Bibliographic analysis of old and modern assays concerning preservation, extraction, assay and interferences.

Results

5HIAA may degrade as soon as urine is excreted. Thus, acids as preservatives (hydrochloric or acetic acid) have to be immediately added. Care should be taken not to decrease the pH under 2. Urine preservative for metanephrine assays

SUBMITTER: Corcuff JB 

PROVIDER: S-EPMC5527357 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature