Bypassing the Restriction System To Improve Transformation of Staphylococcus epidermidis.

ABSTRACT: Staphylococcus epidermidis is the leading cause of infections on indwelling medical devices worldwide. Intrinsic antibiotic resistance and vigorous biofilm production have rendered these infections difficult to treat and, in some cases, require the removal of the offending medical prosthesis. With the exception of two widely passaged isolates, RP62A and 1457, the pathogenesis of infections caused by clinical S. epidermidis strains is poorly understood due to the strong genetic barrier that precludes the efficient transformation of foreign DNA into clinical isolates. The difficulty in transforming clinical S. epidermidis isolates is primarily due to the type I and IV restriction-modification systems, which act as genetic barriers. Here, we show that efficient plasmid tr

SUBMITTER: Costa SK 

PROVIDER: S-EPMC5527377 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature