Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies.

ABSTRACT: Antibodies have become valuable therapeutic agents for targeting of extracellular proteins in various diseases, including cancer, autoimmunity and cardiovascular disorders. For breast cancer, antibodies targeting the human HER2 have been shown to result in cell growth inhibition both in vitro and in patients with breast tumors. There is evidence to suggest that targeting multiple HER2 epitopes may result in increased growth inhibition making it interesting to find antibodies targeting new epitopes. Here, we report on a new scheme to discover antibodies directed to new epitopes using the extracellular domain of the HER2 as a model. Polyclonal antibodies were generated using recombinant protein fragments and affinity purified fractions of the antibodies were functionally characterized and pr

SUBMITTER: Rockberg J 

PROVIDER: S-EPMC5527853 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature