Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?

ABSTRACT: The use of real-time quantitative polymerase chain reaction (qPCR) in cancer research has become ubiquitous. The relative simplicity of qPCR experiments, which deliver fast and cost-effective results, means that each year an increasing number of papers utilizing this technique are being published. But how reliable are the published results? Since the validity of gene expression data is greatly dependent on appropriate normalisation to compensate for sample-to-sample and run-to-run variation, we have evaluated the adequacy of normalisation procedures in qPCR-based experiments. Consequently, we assessed all colorectal cancer publications that made use of qPCR from 2006 until August 2013 for the number of reference genes used and whether they had been validated. Using even these minimal evalu

SUBMITTER: Dijkstra JR 

PROVIDER: S-EPMC5528534 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature