Development and validation of a microRNA based diagnostic assay for primary tumor site classification of liver core biopsies.

ABSTRACT: Identification of the primary tumor site in patients with metastatic cancer is clinically important, but remains a challenge. Hence, efforts have been made towards establishing new diagnostic tools. Molecular profiling is a promising diagnostic approach, but tissue heterogeneity and inadequacy may negatively affect the accuracy and usability of molecular classifiers. We have developed and validated a microRNA-based classifier, which predicts the primary tumor site of liver biopsies, containing a limited number of tumor cells. Concurrently we explored the influence of surrounding normal tissue on classification. MicroRNA profiling was performed using quantitative Real-Time PCR on formalin-fixed paraffin-embedded samples. 278 primary tumors and liver metastases, representing nine primary tum

SUBMITTER: Perell K 

PROVIDER: S-EPMC5528690 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature