Gelatin nanoparticles enhance delivery of hepatitis C virus recombinant NS2 gene.

ABSTRACT:

Background

Development of an effective non-viral vaccine against hepatitis C virus infection is of a great importance. Gelatin nanoparticles (Gel.NPs) have an attention and promising approach as a viable carrier for delivery of vaccine, gene, drug and other biomolecules in the body.

Aim of work

The present study aimed to develop stable Gel.NPs conjugated with nonstructural protein 2 (NS2) gene of Hepatitis C Virus genotype 4a (HCV4a) as a safe and an efficient vaccine delivery system.

Methods and results

Gel.NPs were synthesized and characterized (size: 150±2 nm and zeta potential +17.6 mv). NS2 gene was successfully cloned and expressed into E. coli M15 using pQE-30 vector. Antigenicity of the recombinant NS2 protein was confirmed by Western blotting to verify the ef

SUBMITTER: Sabet S 

PROVIDER: S-EPMC5528829 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature