A user-friendly, low-cost turbidostat with versatile growth rate estimation based on an extended Kalman filter.

ABSTRACT: For various experimental applications, microbial cultures at defined, constant densities are highly advantageous over simple batch cultures. Due to high costs, however, devices for continuous culture at freely defined densities still experience limited use. We have developed a small-scale turbidostat for research purposes, which is manufactured from inexpensive components and 3D printed parts. A high degree of spatial system integration and a graphical user interface provide user-friendly operability. The used optical density feedback control allows for constant continuous culture at a wide range of densities and offers to vary culture volume and dilution rates without additional parametrization. Further, a recursive algorithm for on-line growth rate estimation has been implemented. The em

SUBMITTER: Hoffmann SA 

PROVIDER: S-EPMC5529016 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature