Tcrd Rearrangement Redirects a Processive Tcra Recombination Program to Expand the Tcra Repertoire.

ABSTRACT: Adaptive immunity depends on diverse T cell receptor repertoires generated by variable, diversity, and joining (V[D]J) recombination. Here, we define the principles by which combinatorial diversity is generated in the murine Tcra repertoire. Tcra and Tcrd gene segments share the Tcra-Tcrd locus, with interspersed V_α and V_δ segments undergoing V_δ-D_δ-J_δ rearrangement in CD4^-CD8^- thymocytes and then multiple rounds of V_α-J_α rearrangement in CD4⁺CD8⁺ thymocytes. We document stepwise, highly coordinated proximal-to-distal progressions of V_α and J_α use on individual Tcra alleles, limiting combinatorial diversity. This behavior is supported by an extended

SUBMITTER: Carico ZM 

PROVIDER: S-EPMC5529038 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature