Comparative Visualization of the RNA Suboptimal Conformational Ensemble In Vivo.

ABSTRACT: When a ribonucleic acid (RNA) molecule folds, it often does not adopt a single, well-defined conformation. The folding energy landscape of an RNA is highly dependent on its nucleotide sequence and molecular environment. Cellular molecules sometimes alter the energy landscape, thereby changing the ensemble of likely low-energy conformations. The effects of these energy landscape changes on the conformational ensemble are particularly challenging to visualize for large RNAs. We have created a robust approach for visualizing the conformational ensemble of RNAs that is well suited for in vitro versus in vivo comparisons. Our method creates a stable map of conformational space for a given RNA sequence. We first identify single point mutations in the RNA that maximally sample suboptimal conforma

SUBMITTER: Woods CT 

PROVIDER: S-EPMC5529173 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature