Genetic analyses of HIV env associated with uveitis in antiretroviral-naive individuals.

ABSTRACT:

Objective

To analyze and compare HIV-1 env sequences from the eye to those from the blood of individuals with uveitis attributed to HIV with the goal of gaining insight into the pathogenesis of HIV-associated eye disease.

Design

A prospective case series of five HIV-infected antiretroviral-naive individuals with uveitis negative for other pathogens.

Methods

RNA from blood plasma and ocular aqueous humor was reverse transcribed using random hexamers. HIV env C2-V5 (HXB2: 6990-7668) sequences were generated by single-genome amplification using nested polymerase chain reaction followed by bidirectional Sanger sequencing. Sequence analyses by Geneious, Geno2Pheno, N-GLYCOSITE, DIVEIN, and HyPhy evaluated relationships between HIV in plasma and aqueous humor.

Results

SUBMITTER: Williams-Wietzikoski CA 

PROVIDER: S-EPMC5529222 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature