Release of Applied Mechanical Loading Stimulates Intercellular Calcium Waves in Drosophila Wing Discs.

ABSTRACT: Mechanical forces are critical but poorly understood inputs for organogenesis and wound healing. Calcium ions (Ca²⁺) are critical second messengers in cells for integrating environmental and mechanical cues, but the regulation of Ca²⁺ signaling is poorly understood in developing epithelial tissues. Here we report a chip-based regulated environment for microorgans that enables systematic investigations of the crosstalk between an organ's mechanical stress environment and biochemical signaling under genetic and chemical perturbations. This method enabled us to define the essential conditions for generating organ-scale intercellular Ca²⁺ waves in Drosophila wing discs that are also observed in vivo during organ development. We discovered that mechanically indu

SUBMITTER: Narciso CE 

PROVIDER: S-EPMC5529297 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature