Preservation and Significance of Extracellular DNA in Ferruginous Sediments from Lake Towuti, Indonesia.

ABSTRACT: Extracellular DNA is ubiquitous in soil and sediment and constitutes a dominant fraction of environmental DNA in aquatic systems. In theory, extracellular DNA is composed of genomic elements persisting at different degrees of preservation produced by processes occurring on land, in the water column and sediment. Extracellular DNA can be taken up as a nutrient source, excreted or degraded by microorganisms, or adsorbed onto mineral matrices, thus potentially preserving information from past environments. To test whether extracellular DNA records lacustrine conditions, we sequentially extracted extracellular and intracellular DNA from anoxic sediments of ferruginous Lake Towuti, Indonesia. We applied 16S rRNA gene Illumina sequencing on both fractions to discriminate exogenous from endogenou

SUBMITTER: Vuillemin A 

PROVIDER: S-EPMC5529349 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature