ATP-dependent modulation of MgtE in Mg²⁺ homeostasis.

ABSTRACT: Magnesium is an essential ion for numerous physiological processes. MgtE is a Mg²⁺ selective channel involved in the maintenance of intracellular Mg²⁺ homeostasis, whose gating is regulated by intracellular Mg²⁺ levels. Here, we report that ATP binds to MgtE, regulating its Mg²⁺-dependent gating. Crystal structures of MgtE-ATP complex show that ATP binds to the intracellular CBS domain of MgtE. Functional studies support that ATP binding to MgtE enhances the intracellular domain affinity for Mg²⁺ within physiological concentrations of this divalent cation, enabling MgtE to function as an in vivo Mg²⁺ sensor. ATP dissociation from MgtE upregulates Mg²⁺ influx at both high and low intracellular Mg²⁺ conce

SUBMITTER: Tomita A 

PROVIDER: S-EPMC5529423 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature