4-dimensional functional profiling in the convulsant-treated larval zebrafish brain.

ABSTRACT: Functional neuroimaging, using genetically-encoded Ca²⁺ sensors in larval zebrafish, offers a powerful combination of high spatiotemporal resolution and higher vertebrate relevance for quantitative neuropharmacological profiling. Here we use zebrafish larvae with pan-neuronal expression of GCaMP6s, combined with light sheet microscopy and a novel image processing pipeline, for the 4D profiling of chemoconvulsant action in multiple brain regions. In untreated larvae, regions associated with autonomic functionality, sensory processing and stress-responsiveness, consistently exhibited elevated spontaneous activity. The application of drugs targeting different convulsant mechanisms (4-Aminopyridine, Pentylenetetrazole, Pilocarpine and Strychnine) resulted in distinct spatiotemporal

SUBMITTER: Winter MJ 

PROVIDER: S-EPMC5529444 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature