Molecular profiling of single organelles for quantitative analysis of cellular heterogeneity.

ABSTRACT: Recent developments in Raman spectroscopy instrumentation and data processing algorithms have led to the emergence of Ramanomics - an independent discipline with unprecedented capabilities to map the distribution of distinct molecular groups in live cells. Here, we introduce a method for probing the absolute concentrations of proteins, RNA and lipids in single organelles of live cultured cells by biomolecular component analysis using microRaman data. We found significant cell-to-cell variations in the molecular profiles of organelles, thus providing a physiologically relevant set of markers of cellular heterogeneity. At the same cell the molecular profiles of different organelles can strongly correlate, reflecting tight coordination of their functions. This correlation was significant in W

SUBMITTER: Kuzmin AN 

PROVIDER: S-EPMC5529525 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature