Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant.

ABSTRACT: Pulsed electric fields applied to cells have been used as an invaluable research tool to enhance delivery of genes or other intracellular cargo, as well as for tumor treatment via electrochemotherapy or tissue ablation. These processes involve the buildup of charge across the cell membrane, with subsequent alteration of transmembrane potential that is a function of cell biophysics and geometry. For traditional electroporation parameters, larger cells experience a greater degree of membrane potential alteration. However, we have recently demonstrated that the nuclear/cytoplasm ratio (NCR), rather than cell size, is a key predictor of response for cells treated with high-frequency irreversible electroporation (IRE). In this study, we leverage a targeted molecular therapy, ephrinA1, known to

SUBMITTER: Ivey JW 

PROVIDER: S-EPMC5529610 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature