Combined detection of dickkopf-1 subtype classification autoantibodies as biomarkers for the diagnosis and prognosis of non-small cell lung cancer.

ABSTRACT:

Purpose

This study aims to identify the clinical significance of serum autoantibodies against dickkopf-1 (DKK1) and evaluate their feasibility in the immunodiagnosis and prognosis of non-small cell lung cancer (NSCLC).

Experimental design

Epitope mapping by peptide microarray-based serum screening of NSCLC patients (n=72) and healthy controls (n=16) was performed. Indirect ELISA with peptides was used to measure the serum levels of autoantibodies in 206 NSCLC patients and 99 healthy controls. A 3-year follow-up was monitored to evaluate the correlation between serological levels of autoantibodies and overall survival (OS) and progression-free survival (PFS).

Results

Four highly reactive epitopes were identified, which included peptides 67-84 (Pep A), 37-54 (Pep B), 14

SUBMITTER: Shen L 

PROVIDER: S-EPMC5530063 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature